Construct: shRNA TRCN0000353351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177589.3-2933s21c1
DNA Barcode:
GCTCGACGGTCATGGATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ulk4 (209012)
Gene Description:
unc-51-like kinase 4
Transcript:
RefSeq NM_177589.3 (CURRENT)
Match location:
Position 2933 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGACGGTCATGGATTATA
Hairpin Sequence:
5'-CCGG-GCTCGACGGTCATGGATTATA-CTCGAG-TATAATCCATGACCGTCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGACGGTCATGGATTATACTCGAGTATAATCCATGACCGTCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGACGGTCATGGATTATACTCGAGTATAATCCATGACCGTCGAGC-3'

Other clones with same target sequence:

(none)