Construct: shRNA TRCN0000353387

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021477.5-1713s21c1
DNA Barcode:
GGATCCAGACCTCCGACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbfox1 (268859)
Gene Description:
RNA binding protein, fox-1 homolog (C. elegans) 1
Transcript:
RefSeq NM_021477.5 (CURRENT)
Match location:
Position 1713 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353387)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATCCAGACCTCCGACAAAT
Hairpin Sequence:
5'-CCGG-GGATCCAGACCTCCGACAAAT-CTCGAG-ATTTGTCGGAGGTCTGGATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATCCAGACCTCCGACAAATCTCGAGATTTGTCGGAGGTCTGGATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATCCAGACCTCCGACAAATCTCGAGATTTGTCGGAGGTCTGGATCC-3'

Other clones with same target sequence:

(none)