Construct: shRNA TRCN0000353618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026140.2-1196s21c1
DNA Barcode:
TGTTCTGGACCCTGCATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ears2 (67417)
Gene Description:
glutamyl-tRNA synthetase 2, mitochondrial
Transcript:
RefSeq NM_026140.2 (NON-CURRENT)
Match location:
Position 1196 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTCTGGACCCTGCATATAT
Hairpin Sequence:
5'-CCGG-TGTTCTGGACCCTGCATATAT-CTCGAG-ATATATGCAGGGTCCAGAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTCTGGACCCTGCATATATCTCGAGATATATGCAGGGTCCAGAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTCTGGACCCTGCATATATCTCGAGATATATGCAGGGTCCAGAACA-3'

Other clones with same target sequence:

(none)