Construct: shRNA TRCN0000353651

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006291.2-266s21c1
DNA Barcode:
CGTCTTCACCAAAGGGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFAIP2 (7127)
Gene Description:
TNF alpha induced protein 2
Transcript:
RefSeq NM_006291.2 (NON-CURRENT)
Match location:
Position 266 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353651)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTTCACCAAAGGGAAGAA
Hairpin Sequence:
5'-CCGG-CGTCTTCACCAAAGGGAAGAA-CTCGAG-TTCTTCCCTTTGGTGAAGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTTCACCAAAGGGAAGAACTCGAGTTCTTCCCTTTGGTGAAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTTCACCAAAGGGAAGAACTCGAGTTCTTCCCTTTGGTGAAGACG-3'

Other clones with same target sequence:

  1. TRCN0000138492