Construct: shRNA TRCN0000353676

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000937.3-6242s21c1
DNA Barcode:
CCAGCTCCGTTGTACATAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLR2A (5430)
Gene Description:
RNA polymerase II subunit A
Transcript:
RefSeq NM_000937.3 (NON-CURRENT)
Match location:
Position 6242 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353676)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTCCGTTGTACATAAAT
Hairpin Sequence:
5'-CCGG-CCAGCTCCGTTGTACATAAAT-CTCGAG-ATTTATGTACAACGGAGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTCCGTTGTACATAAATCTCGAGATTTATGTACAACGGAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTCCGTTGTACATAAATCTCGAGATTTATGTACAACGGAGCTGG-3'

Other clones with same target sequence:

(none)