Construct: shRNA TRCN0000353682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017818.3-904s21c1
DNA Barcode:
GCCATTAATGATCCCAAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WRAP73 (49856)
Gene Description:
WD repeat containing, antisense to TP73
Transcript:
RefSeq NM_017818.3 (NON-CURRENT)
Match location:
Position 904 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTAATGATCCCAAGATA
Hairpin Sequence:
5'-CCGG-GCCATTAATGATCCCAAGATA-CTCGAG-TATCTTGGGATCATTAATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTAATGATCCCAAGATACTCGAGTATCTTGGGATCATTAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTAATGATCCCAAGATACTCGAGTATCTTGGGATCATTAATGGC-3'

Other clones with same target sequence:

  1. TRCN0000129903