Construct: shRNA TRCN0000353689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017871.4-576s21c1
DNA Barcode:
TGGTCTACACGGGTGATTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INTS11 (54973)
Gene Description:
integrator complex subunit 11
Transcript:
RefSeq NM_017871.4 (NON-CURRENT)
Match location:
Position 576 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353689)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTCTACACGGGTGATTATA
Hairpin Sequence:
5'-CCGG-TGGTCTACACGGGTGATTATA-CTCGAG-TATAATCACCCGTGTAGACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTCTACACGGGTGATTATACTCGAGTATAATCACCCGTGTAGACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTCTACACGGGTGATTATACTCGAGTATAATCACCCGTGTAGACCA-3'

Other clones with same target sequence:

(none)