Construct: shRNA TRCN0000353742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176096.1-263s21c1
DNA Barcode:
GGTCCTACATTCACTACTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK5RAP3 (80279)
Gene Description:
CDK5 regulatory subunit associated protein 3
Transcript:
RefSeq NM_176096.1 (NON-CURRENT)
Match location:
Position 263 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCCTACATTCACTACTTTC
Hairpin Sequence:
5'-CCGG-GGTCCTACATTCACTACTTTC-CTCGAG-GAAAGTAGTGAATGTAGGACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCCTACATTCACTACTTTCCTCGAGGAAAGTAGTGAATGTAGGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCCTACATTCACTACTTTCCTCGAGGAAAGTAGTGAATGTAGGACC-3'

Other clones with same target sequence:

(none)