Construct: shRNA TRCN0000353783

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024506.3-1013s21c1
DNA Barcode:
GCCATTGGTAAACTCTGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLB1L (79411)
Gene Description:
galactosidase beta 1 like
Transcript:
RefSeq NM_024506.3 (NON-CURRENT)
Match location:
Position 1013 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353783)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTGGTAAACTCTGAGTA
Hairpin Sequence:
5'-CCGG-GCCATTGGTAAACTCTGAGTA-CTCGAG-TACTCAGAGTTTACCAATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTGGTAAACTCTGAGTACTCGAGTACTCAGAGTTTACCAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTGGTAAACTCTGAGTACTCGAGTACTCAGAGTTTACCAATGGC-3'

Other clones with same target sequence:

  1. TRCN0000154750