Construct: shRNA TRCN0000353975

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144808.4-1649s21c1
DNA Barcode:
TCCAGAATCTTGGCCTCCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc39a14 (213053)
Gene Description:
solute carrier family 39 (zinc transporter), member 14
Transcript:
RefSeq NM_144808.4 (CURRENT)
Match location:
Position 1649 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353975)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCAGAATCTTGGCCTCCTAA
Hairpin Sequence:
5'-CCGG-TCCAGAATCTTGGCCTCCTAA-CTCGAG-TTAGGAGGCCAAGATTCTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCAGAATCTTGGCCTCCTAACTCGAGTTAGGAGGCCAAGATTCTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCAGAATCTTGGCCTCCTAACTCGAGTTAGGAGGCCAAGATTCTGGA-3'

Other clones with same target sequence:

  1. TRCN0000110858