Construct: shRNA TRCN0000353998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021287.1-6582s21c1
DNA Barcode:
CGAAGTAGAGAGCCTCATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sptbn2 (20743)
Gene Description:
spectrin beta, non-erythrocytic 2
Transcript:
RefSeq NM_021287.1 (CURRENT)
Match location:
Position 6582 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGTAGAGAGCCTCATCAA
Hairpin Sequence:
5'-CCGG-CGAAGTAGAGAGCCTCATCAA-CTCGAG-TTGATGAGGCTCTCTACTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGTAGAGAGCCTCATCAACTCGAGTTGATGAGGCTCTCTACTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGTAGAGAGCCTCATCAACTCGAGTTGATGAGGCTCTCTACTTCG-3'

Other clones with same target sequence:

  1. TRCN0000089986