Construct: shRNA TRCN0000354131

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054046.4-2952s21c1
DNA Barcode:
CGCTTCCTCTTTGGCGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Def8 (23854)
Gene Description:
differentially expressed in FDCP 8
Transcript:
RefSeq NM_054046.4 (NON-CURRENT)
Match location:
Position 2952 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000354131)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTCCTCTTTGGCGTTAAA
Hairpin Sequence:
5'-CCGG-CGCTTCCTCTTTGGCGTTAAA-CTCGAG-TTTAACGCCAAAGAGGAAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTCCTCTTTGGCGTTAAACTCGAGTTTAACGCCAAAGAGGAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTCCTCTTTGGCGTTAAACTCGAGTTTAACGCCAAAGAGGAAGCG-3'

Other clones with same target sequence:

  1. TRCN0000105900