Construct: shRNA TRCN0000354196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153423.6-492s21c1
DNA Barcode:
GCCCGTCTTAGAGACCTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wasf2 (242687)
Gene Description:
WAS protein family, member 2
Transcript:
RefSeq NM_153423.6 (CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000354196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGTCTTAGAGACCTATAA
Hairpin Sequence:
5'-CCGG-GCCCGTCTTAGAGACCTATAA-CTCGAG-TTATAGGTCTCTAAGACGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGTCTTAGAGACCTATAACTCGAGTTATAGGTCTCTAAGACGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGTCTTAGAGACCTATAACTCGAGTTATAGGTCTCTAAGACGGGC-3'

Other clones with same target sequence:

  1. TRCN0000099312