Construct: shRNA TRCN0000355548

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005360.4-2011s21c1
DNA Barcode:
ACTTACCAGTGTGTTCACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAF (4094)
Gene Description:
MAF bZIP transcription factor
Transcript:
RefSeq NM_005360.4 (NON-CURRENT)
Match location:
Position 2011 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000355548)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTACCAGTGTGTTCACAAA
Hairpin Sequence:
5'-CCGG-ACTTACCAGTGTGTTCACAAA-CTCGAG-TTTGTGAACACACTGGTAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTACCAGTGTGTTCACAAACTCGAGTTTGTGAACACACTGGTAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTACCAGTGTGTTCACAAACTCGAGTTTGTGAACACACTGGTAAGT-3'

Other clones with same target sequence:

(none)