Construct: shRNA TRCN0000355678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020205.2-2662s21c1
DNA Barcode:
ACCGAGTGGCTGATTCCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OTUD7B (56957)
Gene Description:
OTU deubiquitinase 7B
Transcript:
RefSeq NM_020205.2 (NON-CURRENT)
Match location:
Position 2662 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000355678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCGAGTGGCTGATTCCTATA
Hairpin Sequence:
5'-CCGG-ACCGAGTGGCTGATTCCTATA-CTCGAG-TATAGGAATCAGCCACTCGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCGAGTGGCTGATTCCTATACTCGAGTATAGGAATCAGCCACTCGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCGAGTGGCTGATTCCTATACTCGAGTATAGGAATCAGCCACTCGGT-3'

Other clones with same target sequence:

(none)