Construct: shRNA TRCN0000355729

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007050.5-2503s21c1
DNA Barcode:
TGCTTATTCCTACTCCTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRT (11122)
Gene Description:
protein tyrosine phosphatase receptor type T
Transcript:
RefSeq NM_007050.5 (NON-CURRENT)
Match location:
Position 2503 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000355729)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTTATTCCTACTCCTATTA
Hairpin Sequence:
5'-CCGG-TGCTTATTCCTACTCCTATTA-CTCGAG-TAATAGGAGTAGGAATAAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTTATTCCTACTCCTATTACTCGAGTAATAGGAGTAGGAATAAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTTATTCCTACTCCTATTACTCGAGTAATAGGAGTAGGAATAAGCA-3'

Other clones with same target sequence:

(none)