Construct: shRNA TRCN0000355736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004460.2-890s21c1
DNA Barcode:
GATACGGATATACCAGTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAP (2191)
Gene Description:
fibroblast activation protein alpha
Transcript:
RefSeq NM_004460.2 (NON-CURRENT)
Match location:
Position 890 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000355736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATACGGATATACCAGTTATT
Hairpin Sequence:
5'-CCGG-GATACGGATATACCAGTTATT-CTCGAG-AATAACTGGTATATCCGTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATACGGATATACCAGTTATTCTCGAGAATAACTGGTATATCCGTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATACGGATATACCAGTTATTCTCGAGAATAACTGGTATATCCGTATC-3'

Other clones with same target sequence:

(none)