Construct: shRNA TRCN0000355755
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_004439.5-478s21c1
- DNA Barcode:
- TATCCACACATACCAAGTATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA5 (2044)
- Gene Description:
- EPH receptor A5
- Transcript:
- RefSeq NM_004439.5 (NON-CURRENT)
- Match location:
- Position 478 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TATCCACACATACCAAGTATG
- Hairpin Sequence:
-
5'-CCGG-TATCCACACATACCAAGTATG-CTCGAG-CATACTTGGTATGTGTGGATA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTATCCACACATACCAAGTATGCTCGAGCATACTTGGTATGTGTGGATATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATATCCACACATACCAAGTATGCTCGAGCATACTTGGTATGTGTGGATA-3'
Other clones with same target sequence:
(none)
