Construct: shRNA TRCN0000355853

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139245.2-550s21c1
DNA Barcode:
ATCTTCAGGACTACGAGAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPM1L (151742)
Gene Description:
protein phosphatase, Mg2+/Mn2+ dependent 1L
Transcript:
RefSeq NM_139245.2 (NON-CURRENT)
Match location:
Position 550 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000355853)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTTCAGGACTACGAGAAAG
Hairpin Sequence:
5'-CCGG-ATCTTCAGGACTACGAGAAAG-CTCGAG-CTTTCTCGTAGTCCTGAAGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTTCAGGACTACGAGAAAGCTCGAGCTTTCTCGTAGTCCTGAAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTTCAGGACTACGAGAAAGCTCGAGCTTTCTCGTAGTCCTGAAGAT-3'

Other clones with same target sequence:

(none)