Construct: shRNA TRCN0000355897

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003625.2-599s21c1
DNA Barcode:
ACGACATGAAAGATCACTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPFIA2 (8499)
Gene Description:
PTPRF interacting protein alpha 2
Transcript:
RefSeq NM_003625.2 (NON-CURRENT)
Match location:
Position 599 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000355897)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGACATGAAAGATCACTAAG
Hairpin Sequence:
5'-CCGG-ACGACATGAAAGATCACTAAG-CTCGAG-CTTAGTGATCTTTCATGTCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGACATGAAAGATCACTAAGCTCGAGCTTAGTGATCTTTCATGTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGACATGAAAGATCACTAAGCTCGAGCTTAGTGATCTTTCATGTCGT-3'

Other clones with same target sequence:

(none)