Construct: shRNA TRCN0000355899

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024025.1-767s21c1
DNA Barcode:
GAGGGAAGATCCTGGTGCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUSP26 (78986)
Gene Description:
dual specificity phosphatase 26
Transcript:
RefSeq NM_024025.1 (NON-CURRENT)
Match location:
Position 767 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000355899)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGGAAGATCCTGGTGCATT
Hairpin Sequence:
5'-CCGG-GAGGGAAGATCCTGGTGCATT-CTCGAG-AATGCACCAGGATCTTCCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGGAAGATCCTGGTGCATTCTCGAGAATGCACCAGGATCTTCCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGGAAGATCCTGGTGCATTCTCGAGAATGCACCAGGATCTTCCCTC-3'

Other clones with same target sequence:

(none)