Construct: shRNA TRCN0000355921

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006823.2-644s21c1
DNA Barcode:
GAAATTAGCAGGTCTTGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKIA (5569)
Gene Description:
cAMP-dependent protein kinase inhibitor alpha
Transcript:
RefSeq NM_006823.2 (NON-CURRENT)
Match location:
Position 644 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000355921)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATTAGCAGGTCTTGATAT
Hairpin Sequence:
5'-CCGG-GAAATTAGCAGGTCTTGATAT-CTCGAG-ATATCAAGACCTGCTAATTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATTAGCAGGTCTTGATATCTCGAGATATCAAGACCTGCTAATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATTAGCAGGTCTTGATATCTCGAGATATCAAGACCTGCTAATTTC-3'

Other clones with same target sequence:

(none)