Construct: shRNA TRCN0000355949

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130785.3-1031s21c1
DNA Barcode:
TTTGGAGAAAGGCGAACCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPTE2 (93492)
Gene Description:
transmembrane phosphoinositide 3-phosphatase and tensin homolog 2
Transcript:
RefSeq NM_130785.3 (CURRENT)
Match location:
Position 1031 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000355949)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGGAGAAAGGCGAACCAAT
Hairpin Sequence:
5'-CCGG-TTTGGAGAAAGGCGAACCAAT-CTCGAG-ATTGGTTCGCCTTTCTCCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGGAGAAAGGCGAACCAATCTCGAGATTGGTTCGCCTTTCTCCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGGAGAAAGGCGAACCAATCTCGAGATTGGTTCGCCTTTCTCCAAA-3'

Other clones with same target sequence:

(none)