Construct: shRNA TRCN0000356074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003839.2-138s21c1
DNA Barcode:
TGTACCAGTGAGAAGCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFRSF11A (8792)
Gene Description:
TNF receptor superfamily member 11a
Transcript:
RefSeq NM_003839.2 (NON-CURRENT)
Match location:
Position 138 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTACCAGTGAGAAGCATTAT
Hairpin Sequence:
5'-CCGG-TGTACCAGTGAGAAGCATTAT-CTCGAG-ATAATGCTTCTCACTGGTACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTACCAGTGAGAAGCATTATCTCGAGATAATGCTTCTCACTGGTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTACCAGTGAGAAGCATTATCTCGAGATAATGCTTCTCACTGGTACA-3'

Other clones with same target sequence:

(none)