Construct: shRNA TRCN0000356091

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030949.2-576s21c1
DNA Barcode:
AGAGCTGCTTTCTCGGATAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP1R14C (81706)
Gene Description:
protein phosphatase 1 regulatory inhibitor subunit 14C
Transcript:
RefSeq NM_030949.2 (NON-CURRENT)
Match location:
Position 576 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356091)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCTGCTTTCTCGGATAAG
Hairpin Sequence:
5'-CCGG-AGAGCTGCTTTCTCGGATAAG-CTCGAG-CTTATCCGAGAAAGCAGCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCTGCTTTCTCGGATAAGCTCGAGCTTATCCGAGAAAGCAGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCTGCTTTCTCGGATAAGCTCGAGCTTATCCGAGAAAGCAGCTCT-3'

Other clones with same target sequence:

(none)