Construct: shRNA TRCN0000356128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001668.2-842s21c1
DNA Barcode:
AGGGCGTATCCTGGATCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARNT (405)
Gene Description:
aryl hydrocarbon receptor nuclear translocator
Transcript:
RefSeq NM_001668.2 (NON-CURRENT)
Match location:
Position 842 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGCGTATCCTGGATCTAAA
Hairpin Sequence:
5'-CCGG-AGGGCGTATCCTGGATCTAAA-CTCGAG-TTTAGATCCAGGATACGCCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGCGTATCCTGGATCTAAACTCGAGTTTAGATCCAGGATACGCCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGCGTATCCTGGATCTAAACTCGAGTTTAGATCCAGGATACGCCCT-3'

Other clones with same target sequence:

(none)