Construct: shRNA TRCN0000356139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021643.3-2830s21c1
DNA Barcode:
GCATCGCACTGTTAGCATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIB2 (28951)
Gene Description:
tribbles pseudokinase 2
Transcript:
RefSeq NM_021643.3 (CURRENT)
Match location:
Position 2830 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCGCACTGTTAGCATTTA
Hairpin Sequence:
5'-CCGG-GCATCGCACTGTTAGCATTTA-CTCGAG-TAAATGCTAACAGTGCGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCGCACTGTTAGCATTTACTCGAGTAAATGCTAACAGTGCGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCGCACTGTTAGCATTTACTCGAGTAAATGCTAACAGTGCGATGC-3'

Other clones with same target sequence:

(none)