Construct: shRNA TRCN0000356144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006852.3-1741s21c1
DNA Barcode:
TAGGGAATACCGGATTCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TLK2 (11011)
Gene Description:
tousled like kinase 2
Transcript:
RefSeq NM_006852.3 (NON-CURRENT)
Match location:
Position 1741 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGGGAATACCGGATTCATAA
Hairpin Sequence:
5'-CCGG-TAGGGAATACCGGATTCATAA-CTCGAG-TTATGAATCCGGTATTCCCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGGGAATACCGGATTCATAACTCGAGTTATGAATCCGGTATTCCCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGGGAATACCGGATTCATAACTCGAGTTATGAATCCGGTATTCCCTA-3'

Other clones with same target sequence:

(none)