Construct: shRNA TRCN0000356251

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000014.4-2148s21c1
DNA Barcode:
ACCAACTCAAAGATTCGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
A2M (2)
Gene Description:
alpha-2-macroglobulin
Transcript:
RefSeq NM_000014.4 (NON-CURRENT)
Match location:
Position 2148 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356251)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAACTCAAAGATTCGTAAA
Hairpin Sequence:
5'-CCGG-ACCAACTCAAAGATTCGTAAA-CTCGAG-TTTACGAATCTTTGAGTTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAACTCAAAGATTCGTAAACTCGAGTTTACGAATCTTTGAGTTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAACTCAAAGATTCGTAAACTCGAGTTTACGAATCTTTGAGTTGGT-3'

Other clones with same target sequence:

(none)