Construct: shRNA TRCN0000356254

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000014.4-1238s21c1
DNA Barcode:
AGGCGTCCCTATACCAAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
A2M (2)
Gene Description:
alpha-2-macroglobulin
Transcript:
RefSeq NM_000014.4 (NON-CURRENT)
Match location:
Position 1238 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356254)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCGTCCCTATACCAAATAA
Hairpin Sequence:
5'-CCGG-AGGCGTCCCTATACCAAATAA-CTCGAG-TTATTTGGTATAGGGACGCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCGTCCCTATACCAAATAACTCGAGTTATTTGGTATAGGGACGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCGTCCCTATACCAAATAACTCGAGTTATTTGGTATAGGGACGCCT-3'

Other clones with same target sequence:

(none)