Construct: shRNA TRCN0000356322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003496.2-2162s21c1
DNA Barcode:
CCAAACTACGGTCCCTTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRRAP (8295)
Gene Description:
transformation/transcription domain associated protein
Transcript:
RefSeq NM_003496.2 (NON-CURRENT)
Match location:
Position 2162 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACTACGGTCCCTTATAT
Hairpin Sequence:
5'-CCGG-CCAAACTACGGTCCCTTATAT-CTCGAG-ATATAAGGGACCGTAGTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACTACGGTCCCTTATATCTCGAGATATAAGGGACCGTAGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACTACGGTCCCTTATATCTCGAGATATAAGGGACCGTAGTTTGG-3'

Other clones with same target sequence:

(none)