Construct: shRNA TRCN0000356455

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005684.4-1091s21c1
DNA Barcode:
ACCTAGGAAACGGGCTAATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR52 (9293)
Gene Description:
G protein-coupled receptor 52
Transcript:
RefSeq NM_005684.4 (NON-CURRENT)
Match location:
Position 1091 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356455)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTAGGAAACGGGCTAATTC
Hairpin Sequence:
5'-CCGG-ACCTAGGAAACGGGCTAATTC-CTCGAG-GAATTAGCCCGTTTCCTAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTAGGAAACGGGCTAATTCCTCGAGGAATTAGCCCGTTTCCTAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTAGGAAACGGGCTAATTCCTCGAGGAATTAGCCCGTTTCCTAGGT-3'

Other clones with same target sequence:

(none)