Construct: shRNA TRCN0000356486

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003551.2-128s21c1
DNA Barcode:
CCATTATCAAACCAGATATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME5 (8382)
Gene Description:
NME/NM23 family member 5
Transcript:
RefSeq NM_003551.2 (NON-CURRENT)
Match location:
Position 128 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356486)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTATCAAACCAGATATTG
Hairpin Sequence:
5'-CCGG-CCATTATCAAACCAGATATTG-CTCGAG-CAATATCTGGTTTGATAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTATCAAACCAGATATTGCTCGAGCAATATCTGGTTTGATAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTATCAAACCAGATATTGCTCGAGCAATATCTGGTTTGATAATGG-3'

Other clones with same target sequence:

(none)