Construct: shRNA TRCN0000356548

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018068.3-962s21c1
DNA Barcode:
ACTGCGTTTGATGGATCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIWIL2 (55124)
Gene Description:
piwi like RNA-mediated gene silencing 2
Transcript:
RefSeq NM_018068.3 (NON-CURRENT)
Match location:
Position 962 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356548)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGCGTTTGATGGATCTATT
Hairpin Sequence:
5'-CCGG-ACTGCGTTTGATGGATCTATT-CTCGAG-AATAGATCCATCAAACGCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGCGTTTGATGGATCTATTCTCGAGAATAGATCCATCAAACGCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGCGTTTGATGGATCTATTCTCGAGAATAGATCCATCAAACGCAGT-3'

Other clones with same target sequence:

(none)