Construct: shRNA TRCN0000356599

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000674.2-1567s21c1
DNA Barcode:
GTCCCTCCACTAGGAGTTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADORA1 (134)
Gene Description:
adenosine A1 receptor
Transcript:
RefSeq NM_000674.2 (NON-CURRENT)
Match location:
Position 1567 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356599)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCCTCCACTAGGAGTTAAC
Hairpin Sequence:
5'-CCGG-GTCCCTCCACTAGGAGTTAAC-CTCGAG-GTTAACTCCTAGTGGAGGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCCTCCACTAGGAGTTAACCTCGAGGTTAACTCCTAGTGGAGGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCCTCCACTAGGAGTTAACCTCGAGGTTAACTCCTAGTGGAGGGAC-3'

Other clones with same target sequence:

(none)