Construct: shRNA TRCN0000356734

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005795.4-2306s21c1
DNA Barcode:
GGGCATGATTCTACCCTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CALCRL (10203)
Gene Description:
calcitonin receptor like receptor
Transcript:
RefSeq NM_005795.4 (NON-CURRENT)
Match location:
Position 2306 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356734)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGCATGATTCTACCCTTATT
Hairpin Sequence:
5'-CCGG-GGGCATGATTCTACCCTTATT-CTCGAG-AATAAGGGTAGAATCATGCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGCATGATTCTACCCTTATTCTCGAGAATAAGGGTAGAATCATGCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGCATGATTCTACCCTTATTCTCGAGAATAAGGGTAGAATCATGCCC-3'

Other clones with same target sequence:

(none)