Construct: shRNA TRCN0000356780

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001704.2-2979s21c1
DNA Barcode:
CCGATGCATCCCATACGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRB3 (577)
Gene Description:
adhesion G protein-coupled receptor B3
Transcript:
RefSeq NM_001704.2 (NON-CURRENT)
Match location:
Position 2979 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356780)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGATGCATCCCATACGAAAT
Hairpin Sequence:
5'-CCGG-CCGATGCATCCCATACGAAAT-CTCGAG-ATTTCGTATGGGATGCATCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGATGCATCCCATACGAAATCTCGAGATTTCGTATGGGATGCATCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGATGCATCCCATACGAAATCTCGAGATTTCGTATGGGATGCATCGG-3'

Other clones with same target sequence:

  1. TRCN0000439653