Construct: shRNA TRCN0000356808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001837.3-1025s21c1
DNA Barcode:
CCTTCTCTCTTCCTATCAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCR3 (1232)
Gene Description:
C-C motif chemokine receptor 3
Transcript:
RefSeq NM_001837.3 (NON-CURRENT)
Match location:
Position 1025 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTCTCTTCCTATCAATC
Hairpin Sequence:
5'-CCGG-CCTTCTCTCTTCCTATCAATC-CTCGAG-GATTGATAGGAAGAGAGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTCTCTTCCTATCAATCCTCGAGGATTGATAGGAAGAGAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTCTCTTCCTATCAATCCTCGAGGATTGATAGGAAGAGAGAAGG-3'

Other clones with same target sequence:

(none)