Construct: shRNA TRCN0000356840

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012125.3-2732s21c1
DNA Barcode:
TTGTTAACGAGACTGATATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHRM5 (1133)
Gene Description:
cholinergic receptor muscarinic 5
Transcript:
RefSeq NM_012125.3 (NON-CURRENT)
Match location:
Position 2732 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356840)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTTAACGAGACTGATATTC
Hairpin Sequence:
5'-CCGG-TTGTTAACGAGACTGATATTC-CTCGAG-GAATATCAGTCTCGTTAACAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTTAACGAGACTGATATTCCTCGAGGAATATCAGTCTCGTTAACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTTAACGAGACTGATATTCCTCGAGGAATATCAGTCTCGTTAACAA-3'

Other clones with same target sequence:

(none)