Construct: shRNA TRCN0000356939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000796.3-1036s21c1
DNA Barcode:
GAGTGACTGTCCTTGTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DRD3 (1814)
Gene Description:
dopamine receptor D3
Transcript:
RefSeq NM_000796.3 (NON-CURRENT)
Match location:
Position 1036 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGACTGTCCTTGTCTATG
Hairpin Sequence:
5'-CCGG-GAGTGACTGTCCTTGTCTATG-CTCGAG-CATAGACAAGGACAGTCACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGACTGTCCTTGTCTATGCTCGAGCATAGACAAGGACAGTCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGACTGTCCTTGTCTATGCTCGAGCATAGACAAGGACAGTCACTC-3'

Other clones with same target sequence:

(none)