Construct: shRNA TRCN0000356942

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004951.4-323s21c1
DNA Barcode:
TACTAGCCTTGGTCGTCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR183 (1880)
Gene Description:
G protein-coupled receptor 183
Transcript:
RefSeq NM_004951.4 (NON-CURRENT)
Match location:
Position 323 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356942)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTAGCCTTGGTCGTCATTG
Hairpin Sequence:
5'-CCGG-TACTAGCCTTGGTCGTCATTG-CTCGAG-CAATGACGACCAAGGCTAGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTAGCCTTGGTCGTCATTGCTCGAGCAATGACGACCAAGGCTAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTAGCCTTGGTCGTCATTGCTCGAGCAATGACGACCAAGGCTAGTA-3'

Other clones with same target sequence:

(none)