Construct: shRNA TRCN0000356972

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032571.3-775s21c1
DNA Barcode:
ATTCAACTTGAACGTCCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRE3 (84658)
Gene Description:
adhesion G protein-coupled receptor E3
Transcript:
RefSeq NM_032571.3 (NON-CURRENT)
Match location:
Position 775 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000356972)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCAACTTGAACGTCCAAAT
Hairpin Sequence:
5'-CCGG-ATTCAACTTGAACGTCCAAAT-CTCGAG-ATTTGGACGTTCAAGTTGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCAACTTGAACGTCCAAATCTCGAGATTTGGACGTTCAAGTTGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCAACTTGAACGTCCAAATCTCGAGATTTGGACGTTCAAGTTGAAT-3'

Other clones with same target sequence:

(none)