Construct: shRNA TRCN0000357103

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153835.3-1539s21c1
DNA Barcode:
CAAGTTCACCCTCCGACTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRF3 (165082)
Gene Description:
adhesion G protein-coupled receptor F3
Transcript:
RefSeq NM_153835.3 (NON-CURRENT)
Match location:
Position 1539 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357103)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTTCACCCTCCGACTTAC
Hairpin Sequence:
5'-CCGG-CAAGTTCACCCTCCGACTTAC-CTCGAG-GTAAGTCGGAGGGTGAACTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTTCACCCTCCGACTTACCTCGAGGTAAGTCGGAGGGTGAACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTTCACCCTCCGACTTACCTCGAGGTAAGTCGGAGGGTGAACTTG-3'

Other clones with same target sequence:

(none)