Construct: shRNA TRCN0000357186

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015234.4-1718s21c1
DNA Barcode:
AGTAACTATGATGAGGTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRF5 (221395)
Gene Description:
adhesion G protein-coupled receptor F5
Transcript:
RefSeq NM_015234.4 (NON-CURRENT)
Match location:
Position 1718 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357186)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAACTATGATGAGGTTTAT
Hairpin Sequence:
5'-CCGG-AGTAACTATGATGAGGTTTAT-CTCGAG-ATAAACCTCATCATAGTTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAACTATGATGAGGTTTATCTCGAGATAAACCTCATCATAGTTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAACTATGATGAGGTTTATCTCGAGATAAACCTCATCATAGTTACT-3'

Other clones with same target sequence:

(none)