Construct: shRNA TRCN0000357252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005756.2-2516s21c1
DNA Barcode:
CATTACGGTGGTGGGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRG2 (10149)
Gene Description:
adhesion G protein-coupled receptor G2
Transcript:
RefSeq NM_005756.2 (NON-CURRENT)
Match location:
Position 2516 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357252)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTACGGTGGTGGGATATTT
Hairpin Sequence:
5'-CCGG-CATTACGGTGGTGGGATATTT-CTCGAG-AAATATCCCACCACCGTAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTACGGTGGTGGGATATTTCTCGAGAAATATCCCACCACCGTAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTACGGTGGTGGGATATTTCTCGAGAAATATCCCACCACCGTAATG-3'

Other clones with same target sequence:

(none)