Construct: shRNA TRCN0000357320

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016540.3-1801s21c1
DNA Barcode:
ATCCCTAAGGGAGACCTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR83 (10888)
Gene Description:
G protein-coupled receptor 83
Transcript:
RefSeq NM_016540.3 (NON-CURRENT)
Match location:
Position 1801 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357320)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCCTAAGGGAGACCTAAAT
Hairpin Sequence:
5'-CCGG-ATCCCTAAGGGAGACCTAAAT-CTCGAG-ATTTAGGTCTCCCTTAGGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCCTAAGGGAGACCTAAATCTCGAGATTTAGGTCTCCCTTAGGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCCTAAGGGAGACCTAAATCTCGAGATTTAGGTCTCCCTTAGGGAT-3'

Other clones with same target sequence:

(none)