Construct: shRNA TRCN0000357377

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000840.2-1636s21c1
DNA Barcode:
TGATAAGTCGCGCTATGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRM3 (2913)
Gene Description:
glutamate metabotropic receptor 3
Transcript:
RefSeq NM_000840.2 (CURRENT)
Match location:
Position 1636 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357377)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATAAGTCGCGCTATGATTA
Hairpin Sequence:
5'-CCGG-TGATAAGTCGCGCTATGATTA-CTCGAG-TAATCATAGCGCGACTTATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATAAGTCGCGCTATGATTACTCGAGTAATCATAGCGCGACTTATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATAAGTCGCGCTATGATTACTCGAGTAATCATAGCGCGACTTATCA-3'

Other clones with same target sequence:

(none)