Construct: shRNA TRCN0000357419

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000842.3-4075s21c1
DNA Barcode:
ACGAAGAAACCGACGACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRM5 (2915)
Gene Description:
glutamate metabotropic receptor 5
Transcript:
RefSeq NM_000842.3 (NON-CURRENT)
Match location:
Position 4075 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357419)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGAAGAAACCGACGACAAAT
Hairpin Sequence:
5'-CCGG-ACGAAGAAACCGACGACAAAT-CTCGAG-ATTTGTCGTCGGTTTCTTCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGAAGAAACCGACGACAAATCTCGAGATTTGTCGTCGGTTTCTTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGAAGAAACCGACGACAAATCTCGAGATTTGTCGTCGGTTTCTTCGT-3'

Other clones with same target sequence:

(none)