Construct: shRNA TRCN0000357501

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024012.2-1044s21c1
DNA Barcode:
GCTACTCCAACTCCTTCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HTR5A (3361)
Gene Description:
5-hydroxytryptamine receptor 5A
Transcript:
RefSeq NM_024012.2 (NON-CURRENT)
Match location:
Position 1044 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357501)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACTCCAACTCCTTCTTTA
Hairpin Sequence:
5'-CCGG-GCTACTCCAACTCCTTCTTTA-CTCGAG-TAAAGAAGGAGTTGGAGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACTCCAACTCCTTCTTTACTCGAGTAAAGAAGGAGTTGGAGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACTCCAACTCCTTCTTTACTCGAGTAAAGAAGGAGTTGGAGTAGC-3'

Other clones with same target sequence:

(none)