Construct: shRNA TRCN0000357503

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000872.4-2071s21c1
DNA Barcode:
GAATCCAGTCATCAGCTAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HTR7 (3363)
Gene Description:
5-hydroxytryptamine receptor 7
Transcript:
RefSeq NM_000872.4 (NON-CURRENT)
Match location:
Position 2071 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000357503)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATCCAGTCATCAGCTAATA
Hairpin Sequence:
5'-CCGG-GAATCCAGTCATCAGCTAATA-CTCGAG-TATTAGCTGATGACTGGATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATCCAGTCATCAGCTAATACTCGAGTATTAGCTGATGACTGGATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATCCAGTCATCAGCTAATACTCGAGTATTAGCTGATGACTGGATTC-3'

Other clones with same target sequence:

(none)